Fig. 2
RGD-binding integrins are not required for the CV-A9 infection in the SW480 cells. a Blocking of the CV-A9 infection with antibodies against αV, αVβ5 or β1. The ratio of cells exhibiting a fluorescent signal specific for the virus signal to Hoechst-stained nuclei was determined by a Victor3 multilabel counter. Cells infected in the absence of integrin antibodies (panel on the left) were used as a positive infectivity control (infectivity set to 100 %). Error bars indicate standard deviation counted from three independent experiments with 6 parallel samples. Significance is indicated with an asterisk; *P < 0.05, **P < 0.01, ***P < 0.001. NS = not significant. b αV-antibody blocking in the SW480 and A549 cells. Error bars indicate standard deviation counted from 3 to 6 samples from three separate experiments and significant reduction is indicated with an asterisk; ***P < 0.001. c Fluorescence from cytometry analysis. SW480 cells were incubated with the integrin specific monoclonal antibody against α5β1 and secondary antibody and the cell control shown in a white plot was incubated only with a secondary antibody. The result was analyzed by the Cyflogic program. d The integrin binding assay with αVβ6, αVβ3, α5β1 or BSA (as a control). CV-A9 was stained with polyclonal primary and secondary antibodies. The range from the two parallel samples is shown