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Fig. 2 | Virology Journal

Fig. 2

From: HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway

Fig. 2

The CC domain of tetherin is specifically recognized by HSV-2 gD. a Schematic representation of full-length and deletion mutants of tetherin. The cDNAs of full-length human tetherin and its deletion mutants were cloned into pcDNA3.1(−), respectively. The TM, CC, and GPI domains of tetherin are represented in blue. b Expressions of WT and tetherin mutants were confirmed by western blot using the anti-tetherin polyclonal antibody. c-f Co-immunoprecipitation assays (co-IP) were performed by using the antibodies against tetherin and flag. The tetherin mutants delTM, delCT, delGPI and delCC were cotransfected with gD-flag into 293 T cells, respectively. The lysates were coimmunoprecipitated by the flag antibody and then western blot for tetherin (left panels) or coimmunoprecipitated by the tetherin antibody and then western blot for flag (right panels). c The interaction of gD-flag and the delCT. d The interaction of gD-flag and the delTM. e The interaction of gD-flag and the delGPI. f The interaction of gD-flag and the delCC

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