Expression of cytokine gene candidates during CHIKV infection of 293T cells. The relative expression levels of IL-6, COX-2, MBL, IL1-β, IL-8, PLA-2, and TNF-a during CHIKV infection of 293T cells were analyzed. Transcript levels of candidate genes were measured using quantitative RT-PCR and presented as copies of target transcripts per copy of the GAPDH transcripts. RNA was isolated from CHIKV infected cells at 0, 4, and 7 d.p.i. The bars represent the mean values and the error bars represent the SEM values from six quantitative RT-PCR analyses of two independent cell infection experiments. The relative expression levels of IL-6 and MBL in CHIKV T-SBY infected cells were statistically significantly higher (*p < 0.05) at 7 and 4 d.p.i., respectively, whilst the difference in the relative expression levels of COX-2 was highly significant (**p < 0.001) at 4 d.p.i.