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Fig. 4 | Virology Journal

Fig. 4

From: Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Fig. 4

CHIKV infection of 293T cells. (a) 293T cells were infected with either CHIKV T-SBY or V-181 at MOI of 10. Infected cells at 0, 4, 7, and 10 d.p.i. were collected and stained with antibody against CHIKV and FITC-labeled secondary antibodies to visualize the infected cells at 0, 4, 7, and 10 d.p.i. (b) 293T cells were infected with either CHIKV T-SBY or V-181 as described in panel A. Infected cells were collected and stained with propidium iodide (PI) to visualize the nuclei prior examination under a fluorescence microscope. Foci were observed at 0, 4, 7, and 10 d.p.i. (c) 293T cells were infected as detailed in panel A. Infected cells at 0, 4, 7, and 10 d.p.i. were collected and labeled with an anti-CHIKV monoclonal antibody followed by an appropriated FITC-labeled secondary antibody as well as propidium iodide (PI) to visualize the nuclei prior examination under a fluorescence microscope. Quantification of CHIKV infected cells were analyzed using ImageJ 1.49v software after immunostaining. Three independent experiments were performed and the bars represent the mean values and the error bars represent the SEM values. The amount of CHIKV T-SBY infected cells was statistically significantly higher (*p < 0.05) at 4 and 7 d.p.i. (d) Analysis of cell viability using a classical MTT assay. The 293T cells were exposed with either CHIKV T-SBY or V-181 for 2 h at an MOI of 10. Infected cells were assessed for cell viability during 10 days on a daily basis. The bars represent the mean values and the error bars represent the SEM values from three independent experiments. The difference in cell viability in CHIKV T-SBY infected cells was statistically significant (*p < 0.05) at 4 and 5 d.p.i., whilst it was highly significant (**p < 0.001) at 6 d.p.i.

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