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Fig. 2 | Virology Journal

Fig. 2

From: Epstein-Barr viral microRNAs target caspase 3

Fig. 2

EBV miRNAs repress a full-length luciferase-Caspase 3 3′-UTR reporter. HEK293T cells were co-transfected with the designated luciferase-Caspase 3 reporters and either total synthetic control miRNA duplex (CTL) or an EBV miRNA predicted to base pair with the Caspase 3′-UTR (Table 1 includes all WT and mutant sequences). Firefly/Renilla luciferase ratios were normalized to the same reporter transfected with the negative control miRNA (CTL). a Wild type (WT) Caspase 3 is repressed significantly by nine EBV miRNAs. b WT repression is compared to loss of repression of the relevant mutant for each of the miRNAs that showed the greatest WT repression. In all luciferase assays, mean values were from at least four independent transfections. Error bars, standard deviation; P values from two-tailed Student’s t-tests of noted pairs, *P < 0.0001, **P < 0.01

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