Fig. 1

Caspase 3 has 16 predicted sites for EBV miRNA base pairing, 15 of which align with HITS-CLIP data. HITS-CLIP high-throughput sequencing reads (0 or 1 mismatch; ≥25 nt long) from six biological replicates of Ago-bound RNAs in Jijoye BL cells (unique reads, one color per biological replicate) mapped to scale on the Caspase 3 3′-UTR (1576 nt; RefSeq ID: NM_004346.3). Predicted sites of miRNA seed sequence binding for EBV miRNAs (black) and human miRNAs (purple) are noted below, with non-verifiable EBV miRNA sites in gray. When two sites for a given miRNA are present, they are labeled “A” and “B” from 5′ to 3′, respectively. Sites for BARTs 4, 7, and 22B met our initial HITS-CLIP reproducibility thresholds (three or more experimental replicates, peak height of five or more; [18])