Fig. 6

UL7 protein is involved in regulating the transcriptional activation of the HSV-1 α-4 gene. a Comparison of α-4 transcriptional efficiencies during the proliferetion of UL7-MU (filled circles) and WT viral strains (open boxes). Gene expression levels were detected using absolute real-time RT-PCR. Gene copy numbers were quantified according to the gene RNA standard. b HEK293T cells were co-transfected with pGL-α-4, pGL-UL23 or pGL-UL41 and UL7-WT, UL7-MU or control plasmid for 36 h before luciferase activities were quantified. Data are shown as means ± SD. ^∗∗ P < 0.01. Each group was repeated at least three times. c Vero cells were infected with HSV-1 WT (open boxes) or UL7-MU (filled circles) for 3, 4, 5 and 6 h followed by ChIP assay. The α-4 promoter level was determined using absolute real-time RT-PCR. Gene copy numbers were quantified according to the gene DNA standard. The error bars indicate the standard deviation (SD). Data are shown as the means ± SD (three independent experiments). ^∗∗ P < 0.01