Skip to main content
Fig. 5 | Virology Journal

Fig. 5

From: The mutated tegument protein UL7 attenuates the virulence of herpes simplex virus 1 by reducing the modulation of α-4 gene transcription

Fig. 5

The UL7-MU viral strain exhibits attenuated phenotypes in a latent mouse infection model compared with the WT strain. a BALB/c mice were infected with WT HSV-1, UL7-MU or PBS via the foot pad at a dose of 5x103/10 μl per mouse. The viral load was detected in the CNS of mice challenged with WT (open boxes), UL7-MU (filled circles) or PBS (filled triangles) by absolute real-time RT-PCR. Viral copy numbers were quantified according to the HSV-1 DNA standard pGM-T UL30 plasmid. b The levels of LAT expression in the CNS of mice challenged with WT (open boxes), UL7-MU (filled circles) or PBS (filled triangles), as determined by relative real-time RT-PCR. The graphic indicates the fold change of RNA levels in virus-infected mice compared to PBS-injected mice. The mouse housekeeping gene GAPDH was used to normalize quantities in mouse tissue. Relative quantification was performed by the comparative Ct method (Ct) using RNA from PBS mice as a calibrator. c Viral load detection in the spinal cord of mice challenged with WT (open boxes), UL7-MU (filled circles) or PBS (filled triangles). dThe levels of LAT expression in the spinal cord of mice challenged with WT (open boxes), UL7-MU (filled circles) or PBS (filled triangles). e Viral load detection in the trigeminal nerve of mice challenged with WT (open boxes), UL7-MU (filled circles) or PBS (filled triangles). f The levels of LAT expression in the trigeminal nerves of mice challenged with WT (open boxes), UL7-MU (filled circles) or PBS (filled triangles). Data are shown as the means ± SD (experiments done once in triplicate). P < 0.005; P < 0.01; P < 0.05

Back to article page