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Fig. 1 | Virology Journal

Fig. 1

From: The mutated tegument protein UL7 attenuates the virulence of herpes simplex virus 1 by reducing the modulation of α-4 gene transcription

Fig. 1

Identification of the mutated UL7 gene in the viral strain. a Design of the g-RNA sequences for UL7 gene mutation, with the target sites of the g-RNAs (UL7-1 and UL7-2) labeled in yellow. The fragments amplified by oligo 1 and oligo 2 were used in the SURVEYOR assay. b SURVEYOR detection of the mutated genes. Top: SURVEYOR assay of HSV-1 genomic DNA extracted from HEK293T cells expressing UL7-1 and UL7-2 individually or together infected with HSV-1 (P1); bottom: SURVEYOR assay of HSV-1 genomic DNA extracted from HEK293T cells expressing UL7-1 and UL7-2 individually or together infected with HSV-1 progeny virus (P2). c Identification of the mutated UL7 gene in the viral strain. The UL7 mutant was identified via PCR using UL7-sense and UL7-antisense primers. The mutated UL7 gene is indicated with a red box

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