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Fig. 2 | Virology Journal

Fig. 2

From: Codon optimization of antigen coding sequences improves the immune potential of DNA vaccines against avian influenza virus H5N1 in mice and chickens

Fig. 2

Expression of H5 HA in the C2C12 cell line (mouse myoblasts) transfected with the indicated plasmids: HAw/pCI, K3/pCI, GK/pCI and empty pCI as a negative control. About 200 ng of recombinant H5 HA [A/Bar-Headed Goose/Qinghai/12/05 (H5N1)] was used as a positive control (HA). GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) represents the product of an intrinsic gene, was used as a loading control. Data are representative of at least three independent experiments. L – Page Ruler™Prestaned Protein Ladder with the size of protein bands indicated

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Virology Journal

ISSN: 1743-422X