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Fig. 2 | Virology Journal

Fig. 2

From: Responses of primary human nasal epithelial cells to EDIII-DENV stimulation: the first step to intranasal dengue vaccination

Fig. 2

Delivery of EDIII-D3 into HNEpCs. HNEpCs cultures were treated with various amounts of EDIII-D3 TMC NPs or 25 μg of sEDIII-D3 for 2 days prior to the intracellular staining with a monoclonal anti-EDIII specific antibody. The mean fluorescence intensity (MFI) (a) and the percentage of fluorescence-positive cells (b) were quantitated by flow cytometry. Dot plot analysis of HNEpCs treated with isotype control, EDIII-D3 TMC NPs at 112.5 μg and sEDIII-D3 at 25 μg were shown from one representative out of three independent experiments (c). *, ** and *** indicate significant differences between 25, 75 and 112.5 μg of EDIII-D3 TMC NPs and 25 μg of sEDIII-D3 (P <0.05). Medium, EDIII-D3 TMC NPs (25 μg), EDIII-D3 TMC NPs (75 μg), EDIII-D3 TMC NPs (112.5 μg) and sEDIII-D3 (25 μg)

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