Fig. 2

The comparative analysis of Flavivirus NS1. Comparative analysis of Flavivirus NS1 was performed by CLUSTAL Ω software, available on www.ebi.ac.uk/Tools/msa/clustalo/. The UniprotKB accession numbers for different flaviviruses are DENV-1 P27909, DENV-2 P29991, DENV-3 Q6YMS3, DENV-4 Q2YHF0, JEV P27395, TBEV Q01299, WNV P06935, YFV Q6J3P1, ZIKV Q32ZE1, and KUNJV P14335. The conserved sequences are shown by asterisk (٭), the amino acids that are strongly similar in their properties, are indicated by colon (:), those amino acids that are weakly similar, are represented by dot (.), while the dashes (−) represents the gaps among the sequences. The color-coding represents the different types of amino acids like, Red represents small and hydrophobic amino acid, including aromatic amino acid; Tyrosine. Blue represents acidic amino acids. Magenta represents basic amino acids. Green represents amino acids containing hydroxyl, sulfhydryl, amine groups, and Glycine amino acid. Grey represents unusual amino/imino acids