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Fig. 1 | Virology Journal

Fig. 1

From: A single amino acid substitution alter antigenicity of Glycosylated protein 4 of HP-PRRSV

Fig. 1

Images of peptide microarray. The 15 aa peptide printed on chip with a 14 aa duplicate. Each peptide has two replicates on the chip. GP4 peptide region was highlighted with yellow boxes. After being blocked, pre-staining was done with secondary goat anti-swine IgG (H + L) DyLight680 antibody. Subsequent incubation of peptide array with PRRSV SY0608 specific serum was followed by staining with secondary goat anti-swine and read-out at scanning intensities of 5 and 7 (red). HA and FLAG control peptides framing the peptide microarray were subsequently stained at high intensity

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