Skip to main content
Fig. 4 | Virology Journal

Fig. 4

From: G45R mutation in the nonstructural protein 1 of A/Puerto Rico/8/1934 (H1N1) enhances viral replication independent of dsRNA-binding activity and type I interferon biology

Fig. 4

Expression of type I IFN and NS1 in rX31 infected A549 cells and rX31 growth kinetics. a The expression levels of IFNα, IFNβ and NS1 in A549 cells infected with rX31 virus encoding WT or G45R NS1 at 8 h post infection. rX31 with G45R NS1 induced significantly high level of IFNα, IFNβ and NS1 expression compared to the WT virus. Graphs represent mean ± SEM, * and ** represent the statistically significant difference of mean relative mRNA expression compared to the WT at p < 0.05 and p < 0.01, respectively, as determined by student t-test. b Growth of rX31 viruses encoding PR8 NS1 (WT and G45R). rX31 virus with G45R NS1 also replicated to significantly high titer compared to the WT virus early in infection. ** represents the statistically significant difference of mean titers compared to the WT at p < 0.01, as determined by ANOVA

Back to article page

Virology Journal

ISSN: 1743-422X