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Fig. 3 | Virology Journal

Fig. 3

From: G45R mutation in the nonstructural protein 1 of A/Puerto Rico/8/1934 (H1N1) enhances viral replication independent of dsRNA-binding activity and type I interferon biology

Fig. 3

RIG-I mediated IFNβ-promoter activity in 293 T cells expressing PR8 NS1 (wild type; WT, G45R and R38AK41A; AA), RIG I and IFNβ-promoter luciferase reporter at 24 h post infection. WT and G45R NS1s decreased the luciferase expression in dose-dependent manner by inhibition of RIG-I mediated IFNβ-promoter activity. The double mutant R38AK41A served as a negative control failed to inhibit RIG I mediated luciferase expression via IFNβ-promoter. *** represents the statistically significant difference of mean luciferase activity compared to the WT and G45R at p < 0.001, as determined by ANOVA

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Virology Journal

ISSN: 1743-422X