Fig. 2From: G45R mutation in the nonstructural protein 1 of A/Puerto Rico/8/1934 (H1N1) enhances viral replication independent of dsRNA-binding activity and type I interferon biologydsRNA-RBD NS1 pull down assay. The G45R/NS1 bound to dsRNA with lower affinity than the wild type (WT). The R38AK41A as a negative control failed to bind with dsRNA. The input NS1_RBDs for dsRNA-protein pulled down assay were loaded and run onto 15 % acrylamide gel. The input proteins were detected by Coomassie brilliant blue stainingBack to article page