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Table 1 Result summary of non-ribosomal rPCR and Miseq run

From: Development and evaluation of a non-ribosomal random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens

Virusa Sample ID Sample type Cp values % of enteroviral read % Genome coverage Internal gap length (bp) Mean coverage Accession numbers Pairwise identity (%)
CV-A6 1 RS 22.69 90.2 99.5 0 26542 KX430795 100
85.1 100 0 22630 KX430796
2 TS 28.34 11.2 97.5 0 1173 KX430797 100
10.9 95.3 0 1119 KX430798
3 RS 30.5 7.9 97.7 0 1822 KX430799 100
8.3 97.3 57 2244 KX430800
4 TS 32.06 7.1 75 1625 1328 KX430801 99.96
13.3 96.8 41 3061 KX430802
CV-A10 5 RS 20.92 40.7 99.2 0 11189 KX430803 100
53.8 98 0 12725 KX430804
6 RS 23.59 53.1 97.5 0 17439 KX430805 100
51.9 97.6 0 14086 KX430806
7 RS 26.71 18.2 98 0 4299 KX430807 99.99
14.5 97 0 3820 KX430808
8 TS 33.2 42.4 94 0 12216 KX430809 99.99
30.5 97.2 26 8412 KX430810
CV-A16 9 TS 24.97 83.8 99.7 0 3161 KX430811 100
80.1 99 0 20597 KX430812
10 TS 26.72 2 91 71 475 KX430813 100
2.1 96 0 509 KX430814
11 TS 33.26 4.5 96 0 971 KX430815 100
4.2 86 712 1101 KX430816
EV-A71 12 TS 31.1 0.2 72.5 1447 5.3 KX430817 100
0.3 94 0 52.6 KX430818
  1. aMiseq run was multiplexed. Only run output of relevant samples were shown here; CV-A6: coxsackievirus A6, CV-A10: coxsackievirus A10, CV-A16: coxsackievirus A16 and EV-A71 B5: enterovirus A71 subgenogroup B5; TS: Throat swab; RS: rectal swab
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