Fig. 6
Residue N108 in BTas is important for BFV replication. a HEK293T cells (2.5 × 105) were transfected with 1 μg pBS, pBS-BFV-Y, pBS-BFV-B, pBS-BFV-Y-D108N or pBS-BFV-B-N108D, together with pBFV-LTR-Luc and pCMV-β-gal. Forty-eight hours later, the cells were collected and analyzed using a luciferase activity assay. b-c BHK-21 cells (1.2 × 105) were transfected with 1 μg pBS, pBS-BFV-Y, pBS-BFV-B, pBS-BFV-Y-D108N or pBS-BFV-B-N108D. At 48 h post-transfection, cells were harvested for Western blotting to measure viral protein expression b. Cells were also co-cultured with BFVL cells. Luciferase was measured 48 h post-infection c. d Cf2Th cells (1.5 × 105) were transfected with 2.5 μg pBS, pBS-BFV-Y, pBS-BFV-B, pBS-BFV-Y-D108N or pBS-BFV-B-N108D. Seventy-two hours later, syncytia formation (white arrows) was analyzed by microscopy. All results shown are the average from three independent experiments. Error bars indicate SD. *P < 0.05 (unpaired t test), **P < 0.01 (unpaired t test)