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Table 2 Primers used for construction of the plasmids in this study

From: Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus

Primer

F/R

Primer sequence (5′-3′)

Ranges of nucleotides (nts)

Instruction

PUC-A

F

GTTGTAAAACGACGGCCAGTGAATTCACGTGTGTGTATACTGGGGCGG

1–182

pUC-VD1

R

ATCTGTCACTTATCTTGCACCTGACTCTTGATTGTTGCATGTG

PUC-B

F

ATCTGTCACTTATCTTGCACCTGACTCTTGATTGTTGCATGTG

6361–6366

R

GTTGTAAAACGACGGCCAGTGAATTCACGTGTGTGTATACTGGGGCGG

PUC-C

F

CGAATTCACGTGTGTGTATACTGGGGCGG

1–407

pUC-VD2

R

CGCTCGAGTACCGCCCCC

PUC-D

F

CGCTCGAGTACCGCCCCC

5,615–6,022

R

CGGGATCCACGTGTGTGTATACTGGGGCGG

X-VP-pol

F

CGCTCGAGGAGAATATAGAGAAG

2,536–3,533

pVD1-vpgfp

R

CTCTACTGACCCTGGTACTAAGGAC

VP-Mu

F

GTCCTACGGGGAGGCTTGTTGGTTTTCAATAAAGTCGACTATTAAAAT

2,536–3,533

R

AACAAGCCTCCCGTAGGACTTCCTCCTGGATTAATTCTAG

EGFP

F

CGGGAGGCTTGTTGGTTTTCAATAAAGTGAGCAAGGGCGAGGAG

R

GAATAAAACATTTATAAATTTTAATATTACTTGTACAGCTCGTCCATGC

P5

F

CCGGTACCAATTTATACTTTAAGCC

pVD2-mcp/gfp

R

CGCCCTAGGATATAAACTGAGCTTGTATG

p5-gfp-sv40

F

CGGACTAGTAATTTATACTTTAAGCC

CGGAGCTCGATGAGTTTGGACAAACCAC