Skip to main content
Fig. 6 | Virology Journal

Fig. 6

From: Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus

Fig. 6

Detection of progeny virus DNA by PCR. DpnI-VD1 and DpnI-VD2 were used as gene-specific primers for PCR amplifications. BmN (VD1 + VD2) is the total DNA of BmN cells that was co-transfected with linearized full-length genomic DNA- (VD1, VD2); BmN (vp-gfp) is the total DNA of BmN cells that were co-transfected with the linearized recombinant genomic DNA (VD1-vp-gfp); BmN (mcp-gfp) is the total DNA of BmN cells that were co-transfected with the linearized recombinant genomic DNA (VD2-mcp-gfp); pUC-VD1/p and pUC-VD/p served as templates for the positive control group; BmN cells transfected with pUC119 served as the negative control group. The " + " and " − " denote DpnI-treated and untreated templates

Back to article page