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Fig. 5 | Virology Journal

Fig. 5

From: Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus

Fig. 5

(a) Detection of VP expression by western blotting after co-transfection. BmBDV virus particles served as a positive control (lane 1), BmN cells were transfected with plasmid pUC119 as a negative control (lane 2), linearized full-length genomic DNA (VD1, VD2) (lane 3), linearized recombinant genomic DNA(VD1-vp-gfp)(lane 4), and linearized recombinant genomic DNA (VD1-mcp-gfp) (lane 5). (b) Detection of GFP expression by western blotting after co-transfection. pIB-N-GFP as a positive control (lane 1), BmN cells were transfected with pUC119 as a negative control (lane 2), linearized full-length genomic DNA ((VD1, VD2) (lane 3), or the linearized recombinant genomic DNA(VD1-vp-gfp) (lane 4) or (VD1-mcp-gfp) (lane 5)

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Virology Journal

ISSN: 1743-422X