|
Expression plasmids with optimized ORFs
|
RNA
|
Virus
|
|---|
|
VP1
|
VP3
|
VP4
|
VP6
|
NS1
|
NS2
|
Transcripts
|
Recovery
|
|---|
|
-
|
-
|
-
|
-
|
-
|
-
|
Capped
|
-
|
|
+
|
+
|
+
|
+
|
+
|
+
|
Capped
|
+
|
|
-
|
+
|
+
|
+
|
+
|
+
|
Capped
|
-
|
|
+
|
-
|
+
|
+
|
+
|
+
|
Capped
|
+
|
|
+
|
+
|
-
|
+
|
+
|
+
|
Capped
|
+
|
|
+
|
+
|
+
|
-
|
+
|
+
|
Capped
|
+
|
|
+
|
+
|
+
|
+
|
-
|
+
|
Capped
|
+
|
|
+
|
+
|
+
|
+
|
+
|
-
|
Capped
|
-
|
|
+
|
-
|
-
|
-
|
-
|
+
|
Capped
|
+
|
|
+
|
+
|
+
|
+
|
+
|
+
|
Uncapped
|
+
|
|
+
|
-
|
+
|
+
|
+
|
+
|
Uncapped
|
-
|
|
+
|
+
|
-
|
+
|
+
|
+
|
Uncapped
|
+
|
|
+
|
+
|
+
|
-
|
+
|
+
|
Uncapped
|
+
|
|
+
|
+
|
+
|
+
|
-
|
+
|
Uncapped
|
+
|
|
+
|
-
|
-
|
-
|
-
|
+
|
Uncapped
|
-
|
|
+
|
+
|
-
|
-
|
-
|
+
|
Uncapped
|
-
|
- BTV expression plasmids with optimized ORFs in the first transfection mix are indicated. Then, monolayers were transfected with 10 full length in vitro synthesized capped or uncapped run-off RNA transcripts. Virus recovery was microscopically determined by CPE and confirmed by IPMA, and indicated as positive (+) or negative (-)
