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Table 3 Requirements of expression plasmids and run-off RNA transcripts for BTV recovery

From: Requirements and comparative analysis of reverse genetics for bluetongue virus (BTV) and African horse sickness virus (AHSV)

Expression plasmids with optimized ORFs RNA Virus
VP1 VP3 VP4 VP6 NS1 NS2 Transcripts Recovery
- - - - - - Capped -
+ + + + + + Capped +
- + + + + + Capped -
+ - + + + + Capped +
+ + - + + + Capped +
+ + + - + + Capped +
+ + + + - + Capped +
+ + + + + - Capped -
+ - - - - + Capped +
+ + + + + + Uncapped +
+ - + + + + Uncapped -
+ + - + + + Uncapped +
+ + + - + + Uncapped +
+ + + + - + Uncapped +
+ - - - - + Uncapped -
+ + - - - + Uncapped -
  1. BTV expression plasmids with optimized ORFs in the first transfection mix are indicated. Then, monolayers were transfected with 10 full length in vitro synthesized capped or uncapped run-off RNA transcripts. Virus recovery was microscopically determined by CPE and confirmed by IPMA, and indicated as positive (+) or negative (-)