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Fig. 1 | Virology Journal

Fig. 1

From: Requirements and comparative analysis of reverse genetics for bluetongue virus (BTV) and African horse sickness virus (AHSV)

Fig. 1

Schematic presentation of plasmids for run-off RNA transcription and protein expression. a cDNA of full genome segments were cloned between the promoter for DNA dependent RNA polymerase of bacteriophage T7 (PT7) and a recognition site of restriction enzyme (RE) SapI, BsmBI, or BsaI to allow run-off RNA transcription. The appropriate RE was selected based on the absence of the recognition sites in the respective genome segment. Alternatively, internal sites were eliminated by silent mutations with respect to the translated amino acid sequence. b Authentic or open reading frames (ORFs) optimized for plasmid stability and eukaryotic expression (see materials and methods) were cloned between the immediate early promoter of human cytomegalovirus (PHCMV) and the polyA signal (polyA) and transcription terminator (TER)

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