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Fig. 4 | Virology Journal

Fig. 4

From: Antiviral activity of Lactobacillus reuteri Protectis against Coxsackievirus A and Enterovirus 71 infection in human skeletal muscle and colon cell lines

Fig. 4

Immunostaining of RD cells infected with (L. reuteri + CA16) mixture. a L. reuteri Protectis bacteria (1011 CFU) were co-incubated with CA16 (105 PFU) for 1 h in 37 °C prior to adding the mixture onto RD cells (105 cells) for another 1 h. A control with RD cells infected with CA16 only was also performed. The monolayers were then washed thrice before methanol fixation and immunostained with anti-CA16 and anti-beta actin antibodies. Nuclei were also stained with DAPI. Images were taken using Olympus IX81 microscope. b Corrected total cell fluorescence (CTCF) (viral signal) was calculated from each cell originated from three random microscopic views. Statistical analysis was performed using Mann–Whitney U test (****, p < 0.0001)

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