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Fig. 3 | Virology Journal

Fig. 3

From: Construction and sequencing of an infectious clone of the goose embryo-adapted Muscovy duck parvovirus vaccine strain FZ91-30

Fig. 3

Experimental strategy used to construct the infectious FZ91-30 MDPV plasmid clone. a The digested MDPV subgenomic fragments were cloned into the pBSKN plasmid respectively and further combined to form the recombinant plasmid pFZ that contained the whole genome of FZ91-30. b Restriction enzyme digestion of pFZ. M: DNA markers. 1: Digestion with SphI. 2: Double digestion with XhoI and BamHI

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