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Table 2 Primers used for luciferase reporter gene vector construct

From: miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2

Name

Sequence (5’-3’)

TM (oC)

Size (bp)

DNM2-3UTR-S

CA CTCGAGAGAAACAGCACCAAGGGAG

62

412

DNM2-3UTR-A

TA GCGGCCGCCAGGCACTTACAGTGGTCAA

  

DNM2-3UTR-Mut1-A

GGACCTGGCATTCCGTGCTCTGGCAGCCGAGGCTG

  

DNM2-3UTR-Mut1-S

GCTGCCAGAGCACGGAATGCCAGGTCCGGAAAACC

  

DNM2-3UTR-Mut2-A

TAGCGGCCGCCGTGCCTTTACAGTGGTCAAACCGA

  

JEV-E-S

CA CTCGAGAACTCAAAGGTGCTGGTC

62

387

JEV-E-A

TA GCGGCCGCACCTCCTGTGGCTAAGAA

  

JEV-NS4B-S

CA CTCGAGGTCTTGACCGTGGTTGGA

60

378

JEV-NS4B-A

TA GCGGCCGCCGTTGTGAGGGTGACTTG

  

Universal primer-S

CCACTTCAGCCAGGAGGA

60

216

Universal primer-A

GGTCCGAAGACTCATTTAGA

  
  1. S Sense primer, A anti-sense primer, the underline indicates additional bases and restriction enzyme recognition sites are bold
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Virology Journal

ISSN: 1743-422X