Fig. 2

JEV genomic RNA is not the target of miR-124. a Computational prediction of potential miR-124 target sites in JEV genomic RNA. Sequence alignments of 10 representative JEV strains. b Effect of miR-124 mimic on the expression of luciferase from reporter constructs. Sequences of predicted target sites in the JEV genome were inserted into the dual luciferase reporter vector. BHK-21 cells were co-transfected with reporter constructs and the miR-124 or nonspecific (NC) miRNA mimics. The luciferase assay was performed 24 h after the transfection. Renilla luciferase values were normalized against firefly luciferase values. NS, not significant