Fig. 2

Mutational frequencies of 5 amino acids using Sanger and MiSeq high-throughput sequencing. The frequencies of 5 sites in gp85 from Sanger and Highthroughput were compared and generated by Weblogo. “Z” indicates deletion, while other capital letters indicate the normal amino acids. The size of the capital indicated the proportion of the amino acid in this position. The bigger the capital, the higher the proportion. Proportion higher than 1 % were retained for analyzing in High-throughput sequencing