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Table 5 Results of inactivation of selected viruses by boiling

From: Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA

Virus Species

Virus Genus (Family)

Cell Line

Days of Incubation

Virus Titers and Boiling Times (Log base 10 of TCID50 ml−1)

0 min

5 min

10 min

African horse sickness virus (Serotype 4)

Orbivirus

(Reoviridae)

Vero

10

7.7

nvd

nvd

African swine fever virus

(Brazil strain)

Asfivirus (Asfarviridae)

Vero

10

5.9

nvd

nvd

Foot-and-mouth disease virus (O1 Campos)

Apthovirus

(Picornaviridae)

IBRS-2

3

7.5

nvd

nvd

Lumpy skin disease virus (Ismalia strain)

Capripoxvirus

(Poxviridae)

FBL

13

7.9

nvd

nvd

Porcine parvovirus

(NVSL strain)

Protoparvovirus

(Parvovirinae)

ST

10

5.9

nvd

nvd

Rinderpest virus

(Kabete O strain)

Morbillivirus

(Paramyxoviridae)

Vero

9

6.4

nvd

nvd

Swine vesicular disease

(UK 27/72 strain)

Enterovirus

(Picornaviridae)

IBRS-2

3

6.8

nvd

nvd

Vesicular exanthema of swine (Serotype A 48)

Vesivirus

(Caliciviridae)

Vero

3

8.9

nvd

nvd

Vesicular stomatitis virus

(Serotype Indiana 1)

Vesiculovirus

(Rhabdoviridae)

Vero

3

5.8

nvd

nvd

  1. No virus detected (nvd); African green monkey kidney cell line (Vero); swine kidney cell line (IBRS-2); fetal bovine lung (FBL); swine testicular cell line (ST); J. A. House, et al., unpublished observations