From: Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA
Sample | TCID50 | NaOH | 65 °C Incubation | NaCl | Post Neutralization Boil | VI | FMD rRT-PCR | CSF rRT-PCR |
---|---|---|---|---|---|---|---|---|
1- FMDV | 6.53x104 | - | - | - | - | + | NT | NT |
2- CSFV | 4.11x105 | - | - | - | - | + | NT | NT |
3- FMDV | 3.51x104 | - | - | - | - | + | NT | NT |
4- CSFV | 2.21x105 | - | - | - | - | + | NT | NT |
5- FMDV | 3.51x104 | - | - | 0.25 Na | - | + | NT | NT |
6- CSFV | 2.21x105 | - | - | 0.25 Na | - | + | NT | NT |
7- FMDV | 3.51x104 | - | - | - | - | + | NT | NT |
8- CSFV | 2.21x105 | - | - | - | - | + | NT | NT |
9- FMDV + SCR | 2.81x104 | - | 60 min | - | 10 min | - | NT | NT |
10- CSFV + SCR | 1.77x105 | - | 60 min | - | 10 min | - | NT | NT |
11- FMDV+ SCR | 2.81x104 | 0.25 N | 60 min | 0.25 N | 10 min | - | NT | NT |
12- CSFV + SCR | 1.77x105 | 0.25 N | 60 min | 0.25 N | 10 min | - | NT | NT |
13- NEG TE | 0 | - | - | - | - | - | NT | NT |
14- NEG Salt | 0 | - | - | 0.25 Na | - | - | NT | NT |
15- NEG Salt | 0 | - | - | 0.25 Na | - | - | NT | NT |
16- NEG MEM | 0 | - | - | - | - | - | NT | NT |
17- FMDV + SCR | 3.98x106 | - | - | - | - | + | + | NT |
18- FMDV + SCR | 3.98x106 | - | 60 min | - | - | + | + | NT |
19- FMDV + SCR | 3.98x106 | - | - | - | 5 min | - | + | NT |
20- FMDV + SCR | 3.98x106 | - | - | - | 10 min | - | + | NT |
21- FMDV + SCR | 3.98x106 | 0.25 N | 60 min at 25 °C | 0.25 N | - | - | - | NT |
22- FMDV + SCR | 3.98x106 | 0.25 N | 60 min | 0.25 N | 10 min | - | - | NT |
23- TE | 0 | - | - | - | - | - | - | NT |
24- CSFV + SCR | 2.23x104 | - | - | - | - | + | NT | + |
25- CSFV + SCR | 2.23x104 | - | 60 min | - | - | + | NT | + |
26- CSFV + SCR | 2.23x104 | - | - | - | 5 min | - | NT | + |
27- CSFV + SCR | 2.23x104 | - | - | - | 10 min | - | NT | + |
28- CSFV + SCR | 2.23x104 | 0.25 N | 60 min at 25 °C | 0.25 N | - | - | NT | + |
29- CSFV + SCR | 2.23x104 | 0.25 N | 60 min | 0.25 N | 10 min | - | NT | - |
30- TE | 0 | - | - | - | - | - | NT | - |