Fig. 2
From: Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA
Plasmid DNA integrity following alkaline and heat treatment. Imaging of pGEM Teasy plasmid DNA samples as visualized by gel electrophoresis. Safety treated DNA samples are shown following incubation in 0.25 N NaOH at 65 °C for 30, 45 and 60 min followed by neutralization of pH and incubation at 100 °C for 10 min (lanes 2, 3 and 4, respectively). Controls consisted of heat only treated DNA (lane 1, 60 min at 65 °C and 10 min at 100 °C in 250 mM NaCl and TE pH 8.0), or untreated plasmid in TE or 250 mM NaCl (lanes 5 and 6, respectively). Molecular weight markers are 10 kbp, 4 kbp, 2 kbp, 800 bp and 400 bp (lane M)