Table 6 Comparison of custom and FTD assay in regard to sample testing
Custom assay | FTD assay | |
|---|---|---|
Viral Pathogens | Flu A, Influenza | Flu A, Influenza |
A(H1N1)pdm09, Flu B | A(H1N1)pdm09, Flu B | |
HPIV - 1, 2, 3, 4 | HPIV - 1, 2, 3, 4 | |
HCoV OC43, 229E, NL63, | HCoV OC43, 229E, NL63, | |
HKU1, | HKU1, | |
RSV A/B | RSV A/B | |
HMPV A/B | HMPV A/B | |
EV | EV | |
HPeV | HPeV | |
HRV | HRV | |
HAdV | HAdV | |
HBoV | HBoV | |
-- | M. pneu | |
Sample volume | 200 μl | 200 μl |
Elution volume | 55 μl | 55 μl |
PCR volume | 25 μl | 25 μl |
Principle of detection | Fluroscence probe based | Fluroscence probe based |
Hands on time | 50 min | 50 min |
Time to result | 3.5 h | 3.01 h |
Total cost per sample | INR 1500 | INR 4300 |
Ease of Handling | + | + |
Equipment | NucliSENS EasyMAG, ABI 7500 DX Fast | NucliSENS EasyMAG, ABI 7500 DX Fast |
No. of PCR tubes used per sample | 6 | 5 |
Primers & probes | Custom assay (ABI) | FTD |
Number of targets/tube | 3 | 4 |
PCR reaction volumes | ||
2X RT-PCR buffer | 12.5 μl | 12.5 μl |
25X RT-PCR enzyme | 1.0 μl | 1.0 μl |
Primer probe mix/tube | 1.8 μl | 1.5 μl |
Nucleic acid | 9 μl | 10 μl |
Nuclease free water | 0.7 μl | -- |