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Fig. 3 | Virology Journal

Fig. 3

From: Isolation of dengue virus serotype 4 genotype II from a patient with high viral load and a mixed Th1/Th17 inflammatory cytokine profile in South Brazil

Fig. 3

DENV-4 LRV13/422 infection profile in vitro. C6/36 and Huh7.5 cells were seeded in 96-well plates (1 × 104 cells/well) and were infected with DENV-4 (TVP/360 and LRV13/422) at a MOI of 0.1 in Leibovitz (L-15) or DMEM/F12 medium, respectively, without FBS for 1 h and 30 min. The inocula were then removed, and medium supplemented with 5 % (C6/36) or 10 % (Huh7.5) FBS in a final volume of 200 μL. After 24 h, 48 h or 72 h post-infection, cells were fixed and permeabilized, and the extent of infection was analyzed in an Operetta system using 4G2 primary antibody and an Alexa 633-conjugated secondary antibody (a). Additionally, viral titration of C6/36 and Huh7.5 cell supernatants was performed to analyze the secretion of viable viral particles (b). Data were analyzed using a two-way ANOVA followed by Bonferroni’s test. Values represent the means ± SD of the results of three independent experiments. * P <0.05; ** P <0.01

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