Antibodies against Lewis antigens inhibit the binding of human norovirus GII.4 virus-like particles to saliva but not to intestinal Caco-2 cells

Table 2 VLP binding blockade by NoV GII.4 variant-specific mAbs

	NoV GII.4 variant VLP binding to D-Caco-2 cells^a
mAb	GII.4 1999 (v2)	GII.4-2004 (v0)	GII.4-2006b
none	23 ± 5	17 ± 2	22 ± 3
av2 mAb	6 ± 2 (74 %)	-	-
av0 mAb	-	3 ± 1 (82 %)	-
3C3G3 mAb	-	-	10 ± 2 (54.5 %)

^a Mean values ± SD of fluorescent cell counts in ten microscopic fields (40X). The blocking of VLP binding to D-Caco-2 cells was performed with mAbs by preincubating the different VLPs for 1 h with the test antibodies diluted to 1:100 in PBS or with PBS alone for the negative control. Blocking percentages are indicated between parentheses

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