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Fig. 7 | Virology Journal

Fig. 7

From: Antibodies against Lewis antigens inhibit the binding of human norovirus GII.4 virus-like particles to saliva but not to intestinal Caco-2 cells

Fig. 7

Heterogeneous expression of HBGAs on D-Caco-2 cell surface. a Immunofluorescence assay on D-Caco-2 cells fixed with methanol. Lewis and H antigens were detected by anti-Lea (images not shown), Leb, Lex, Ley, H1 and H2 antibodies, followed by FITC-labeled anti–mouse IgG. Cells were counterstained with Evans blue (red fluorescence). HBGA expression in D-Caco-2 cells was evaluated by counting immunofluorescent cells in 10 microscopic fields (40X). (a and d) Weak expression of both Leb and H1 antigen (score 1+), respectively. (b and c) Strong expression of Lex and Ley (score 2+). (e) Very strong H2 antigen expression on D-Caco-2 cell surface (score 3+). b Mean counts of 10 microscopic fields (40X) with standard deviations of D-Caco-2 cells immunostained with anti-Lea, Leb, Lex, Ley, H1 and H2 antibodies (fluorescent cells/microscopic field). All differences were statistically significant (p < 0.01)

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