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Fig. 5 | Virology Journal

Fig. 5

From: Pre-immune state induced by chicken interferon gamma inhibits the replication of H1N1 human and H9N2 avian influenza viruses in chicken embryo fibroblasts

Fig. 5

Relative expression of type I interferons and interferon-stimulated genes following H9N2 infection in CEFs. Twenty-four hours after transfection with pcDNA-IFN-γ, CEFs were infected with 0.01 MOI of H9N2 virus. At 0, 6, 12, 24, and 48 hpi, the total RNA content of CEFs was extracted and analyzed by real-time RT-PCR, as described in Methods. The expression levels of chIFN-α (a), chIFN-β (b), chMx (c), chPKR (d), and chOAS (e), and chRNaseL (f) were assessed. Statistical significance was determined by two-way ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001)

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Virology Journal

ISSN: 1743-422X