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Fig. 6 | Virology Journal

Fig. 6

From: Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China

Fig. 6

Schematic representation for epitope mapping of the monoclonal antibody MAb-TZSV-NP(S18). Immunoblotting was performed for assays. The produced MAb-TZSV-NP(S18) and MAb-TZSV-NP(S15) were used to react with the bacterial-expressed complete nucleocapsid protein (NP) open reading frame (ORF) of Tomato zonate spot virus (TZSV) and truncated TZN1–87. The truncated TZN1–52, TZN1–38, TZN84–86, TZN81–86 and TZN78–86 were expressed by fusing with enhanced green fluorescent protein (eGFP) and verified by the antiserum RAs-GFP [40]. The amino acid residues of TZN84–86, TZN81–86 and TZN78–86 are shown in parentheses. The expressed portions of TZSV NP are indicated by blue boxes. The eGFP ORF is indicated by green boxes. The restriction enzyme sites used for constructions are shown

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