Fig. 4

Identification of Calla lily chlorotic spot virus (CCSV), Tomato zonate spot virus (TZSV) and Tomato necrotic spot associated virus (TNSaV) by reverse transcription-polymerase chain reaction (RT-PCR). Total RNAs extracted from the virus-infected leaves of Nicotiana benthamiana plants were used for assays. Total RNA from healthy N. benthamiana plant (H) was used as a negative control. The TZSV-specific primer pair TZ-f/TZ-r (a), the CCSV-specific primer pair CC-f/CC-r (b) and the TNSaV-specific primer pair TN-f/TN-r (c) can be used to amplify 237, 350 and 305 bp of DNA fragments, respectively