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Fig. 3 | Virology Journal

Fig. 3

From: Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China

Fig. 3

Serological reaction analyses of the produced monoclonal antibodies MAb-TZSV-NP(S15) (a) and MAb-TZSV-NP(S18) (b). The leaf crude extracts of Nicotiana benthamiana plants separately infected with Alstroemeria necrotic streak virus (ANSV), Calla lily chlorotic spot virus (CCSV), Capsicum chlorosis virus (CaCV), Chrysanthemum stem necrosis virus (CSNV), Groundnut bud necrosis virus (GBNV), Groundnut chlorotic fan-spot virus (GCFSV), Groundnut ringspot virus (GRSV), Hippeastrum chlorotic ringspot virus (HCRV), Impatiens necrotic spot virus (INSV), Iris yellow spot virus (IYSV), Melon yellow spot virus (MYSV), Tomato chlorotic spot virus (TCSV), Tomato spotted wilt virus (TSWV), Tomato yellow ring virus (TYRV), Tomato zonate spot virus (TZSV), Watermelon bud necrosis virus (WBNV) or Watermelon silver mottle virus (WSMoV) were used for indirect enzyme-linked immunosorbent assay (upper panels) and immunoblotting (lower panels). The crude extract of a healthy N. benthamiana leaf (H) was used as the negative control

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