Fig. 2
Analyses of the produced monoclonal antibodies reacted with Tomato zonate spot virus (TZSV) and other Watermelon silver mottle virus (WSMoV)-serogroup members. MAb-TZSV-NP(S15) (a and b) and MAb-TZSV-NP(S18) (c and d) were used at the 10−4 dilution and 10−3 dilution, respectively, in indirect enzyme-linked immunosorbent assay (a and c) and immunoblotting (b and d). The leaf crude extracts of Nicotiana benthamiana plants separately infected with Calla lily chlorotic spot virus (CCSV), Tomato necrotic spot associated virus (TNSaV), TZSV or WSMoV were used for serological assays. The crude extracts of a healthy N. benthamiana leaf (H) was used as the negative control. The plant ribulose bisphosphate carboxylase/oxygenase (rubisco) is shown by Ponceau S staining as a control in immunoblotting to indicate the loading quantity