Fig. 1
Titration assays of the produced monoclonal antibodies (MAbs). MAb-TZSV-NP(S15) (a and b) and MAb-TZSV-NP(S18) (c and d) against the nucleocapsid protein (NP) of Tomato zonate spot virus (TZSV) were assayed by indirect enzyme-linked immunosorbent assay. a and c the crude extracts from leaves of healthy (H, white box) and TZSV-13YV639-infected (blue box) Nicotiana benthamiana plants were used at a 1/50 dilution for testing the titers of the produced MAbs. The dilutions of MAbs are indicated at the X axis. b and d a 10-fold serial dilution of the purified bacterial-expressed recombinant NP (rNP, red box) of TZSV was used for analyzing the sensitivity of the produced MAbs. The amounts of rNP are indicated at the X axis. MAb-TZSV-NP(S15) was used at a 10−4 dilution and MAb-TZSV-NP(S18) was used at a 10−3 dilution for reacting with rNP. The absorbance at 405 nm (A405) recorded by a Model 680 microplate reader (Bio-Rad) at 30 min after colorization is shown at the Y axis