Fig. 5

Double immunostaining of ICP8 and RNA Pol II Ser2P. Experimental group cells were infected with HSV-1 17+ strain for 6 h, then fixed for IF. In first line, signals are captured from red channel, which were stained with anti-ICP8 monoclonal antibody; Second line, signals are captured from green channel, which were stained with anti-RNA Pol II Ser2P polyclonal antibody; Third line, images are merged to examine colocalization situation of two color signals; Fourth line, partial enlarged detail of figures in the third line are shown; Fifth line, images from the third line were analyzed, which were done with Image-Pro Plus 6.0 software (USA). a-c: Cells were not infected, images are captured with STED microscopy. d-h: Cells were infected at a MOI of 0.1 PFU/cell, images are captured with STED microscopy and then analyzed. i-m: Cells were infected at a MOI of 5 PFU/cell, images are captured with STED microscopy and then analyzed. Host cell nucleus are indicated with white dotted lines. g, l: Higher zooms of regions inside the white squares are shown. Scale bars, 2.5 μm. Rr: correlation coefficient; R: overlapping coefficient