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Fig. 4 | Virology Journal

Fig. 4

From: Visualizing the replicating HSV-1 virus using STED super-resolution microscopy

Fig. 4

ICP8 signals is highly related to the replicating HSV-1 genome. All cells were infected with HSV-1 17+ strain and at a MOI of 5 PFU/cell for 6 h, then prepared for IF-FISH. a-c: At early stage of HSV-1 replication, images are captured with STED microscopy. d, e: Higher zooms of regions inside C are shown, which are indicated by white squares. f: Analysis results of C is shown. g-i: At late stage of HSV-1 replication, images are captured with STED microscopy. j, k: Higher zooms of regions inside I are shown, which are indicated by white squares. l: Analysis results of I is shown. Host cell nucleus are indicated with white dotted lines. Scale bars, 2.5 μm. Rr: correlation coefficient; R: overlapping coefficient; m2: colocalization coefficient

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