Fig. 1
From: Visualizing the replicating HSV-1 virus using STED super-resolution microscopy
Resolution of STED microscopy is higher than confocal microscopy. All cells were infected with HSV-1 17+ strain for 6 h, then prepared for FISH. In first line, signals are captured from red channel, which were hybridized with Biotin labeled probe; Second line, signals are captured from green channel, which were hybridized with DIG labeled probe; Third line, images are merged to examine colocalization situation of two color signals; Fourth line, partial enlarged detail of figures in the third line are shown; Fifth line, images from the third line were analyzed, which were done with Image-Pro Plus 6.0 software (USA). a: A brief description of HSV-1 genome structure. Relative to HSV-1 genome, probe locates at the two terminus, which contains TRL, IRL, IRS, TRS, US and partial UL region. The same probe is labeled with either DIG or Biotin to generate two different colors. b-e, f-k: Cells were infected at a MOI of 0.1 PFU/cell. At early stage of HSV-1 replication, images are captured with confocal microscopy and STED microscopy, respectively, and then analyzed. l-o, p-u: Cells were infected at a MOI of 5 PFU/cell. At late stage of HSV-1 replication, images are captured with confocal microscopy and STED microscopy, respectively, and then analyzed. Host cell nucleus are indicated with white dotted lines. i, j, s, t: Higher zooms of regions inside the white rectangles are shown. Scale bars, 2.5 μm. Rr: correlation coefficient; R: overlapping coefficient; k: antigen contribution