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Fig. 9 | Virology Journal

Fig. 9

From: A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host

Fig. 9

The enhancing effect of α-tocopherol and the inhibitory effects of anti-HCV drugs on HCV replication. Huh7 (a) and HLCs (b) were treated with 0, 1, 5 μM α-tocopherol 24 h prior to infection with HCVser and assayed for HCV viral load. For anti-viral treatment, HLCs were either infected with HCVcc or HCVser (n = 3) for 24 h prior to the treatment with ribavirin, Interferon-α or sofosbuvir for 7 d. Copies of HCV RNA in lysates were quantified by real-time PCR (c, d). Infected HLCs were assayed for inflammatory markers (e). For long-term maintenance of HCVcc in HLCs, the supernatants of the infected HLCs were measured for viral load up to 6 months (f)

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Virology Journal

ISSN: 1743-422X