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Fig. 7 | Virology Journal

Fig. 7

From: A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host

Fig. 7

Production of HCVcc (JFH-1) in HLCs and the viral propagation kinetics. HLCs were either mock transfected (a) or transfected with JFH-1 RNA to produce HCVcc had induced cytopathic effect, cell aberrations (CPE) on day 7 (b) and syncytial formation (c) on day 14. In comparison, Huh7 cells were either mock transfected (d) or transfected with JFH-1 RNA on day 7 (e) and 14 (f). Cell viability assay using MTT in HLCs (g) and Huh7 (h) corresponded to the MOI of HCVcc. The kinetics of HCV production in the culture medium were investigated using Abbott real-time HCV assay (i); while the kinetics of HCV replication were investigated using stand-specific real-time RT-PCR (j) in Huh7, HLCs and HepaRG cells

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Virology Journal

ISSN: 1743-422X