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Fig. 5 | Virology Journal

Fig. 5

From: A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host

Fig. 5

The detection of function CYP450 isoenzymes in HLCs. Mature HLCs were investigated for CYP450 expression, protein levels and activities. HLC, HepaRG and primary hepatocyte were studied for the expression of CYP450 isoenzymes and presented as fold changes over the undifferentiated iPS cells (a). The CYP450 induction with the cocktail of inducers was evaluated and presented as fold changes over the corresponding untreated cells (b). * and ** represented statistical different data with a p value <0.05 or <0.01 respectively. The levels of CYP3A4 (c), CYP2B6 (d), CYP1A1 (e), CYP2C9 (f) and CYP2E1 (g) in untreated HLCs and inducer-treated HLCs were measured. Bile canaliculi marker (MRP2) was observed in HLCs (h), but not in human fibroblasts (i). The CYP450 activities (j) were assayed and presented as a relative light unit (RLU) over the corresponding untreated cells

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Virology Journal

ISSN: 1743-422X