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Fig. 2 | Virology Journal

Fig. 2

From: A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host

Fig. 2

Identifications of pluripotent markers in human iPS cells derived from hMSCs. Newly established iPS cells were stained with monoclonal antibodies against pluripotent protein markers, e.g., Nanog, Oct-4, Sox2, SSEA4, Tra-1-60 and Tra-1-81. The iPS cells were counter stained with DAPI and visualized under a fluorescent microscope. Human fibroblast served as negative controls (a). To elucidate endogenous expressions of pluripotent markers in human iPS cells, specific primers were designed to detect the endogenous expression of Oct4, Sox2, Klf4, c-MYC, Nanog, REX1, GDF3, DNM3TB and UTF1 (b). The iPS cells (lane 1) expressed all pluripotent genes similarly to those of hES (BG01V: lane 2). Normal hMSC expressed only Klf4 and C-MYC (lane 3). PCR products from mouse embryonic fibroblast sample (lane 4) and NTC (lane 5) served as negative controls

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Virology Journal

ISSN: 1743-422X