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Table 3 Primers used in real time PCR

From: Avian leukosis virus subgroup J induces its receptor--chNHE1 up-regulation

Gene

Primer

Primer sequence

Size of PCR product

gp85

Pf

GTCACTAATGTTACTGCTTGCG

434 bp

Pr

GAACTACACCAATTAGAAAG

chNHE1

Pf

CTGAACAAACAGCACCACAAC

291 bp

Pr

GAGGAGGAAGAGGAAGAAGATG

GAPDH

Pf

GGTGGTGCTAAGCGTGTTA

179 bp

Pr

CCCTCCACAATGCCAA

  1. The gp85 primers were used for detecting the mRNA level expression of ALV-J