Table 2 The primer sequences and product size of MY09/11, GP5+/6+ and SPF1/2
Primer | sequence | Size (bp) |
|---|---|---|
MY09/11 | F: 5ʹ- CGTCCMARRGGAWACTGATC- 3ʹ | 450 |
|  | R: 5ʹ- GCMCAGGGWCATAAYAATGG- 3ʹ |  |
GP5+/6+ | F: 5ʹ- TTTGTTACTGTGGTAGATACTAC- 3ʹ | 150 |
|  | R: 5ʹ- GAAAATAAACTGTAAATCATATTC - 3ʹ |  |
SPF1A | F: 5ʹ- GCiCAGGGiCACAATAATGG - 3ʹ | 65 |
SPF1B | R: 5ʹ- GCiCAGGGiCATAACAATGG - 3ʹ |  |
SPF1C | F: 5′- GCiCAGGGiCATAATAATGG - 3′ |  |
SPF1D | R: 5′- GCiCAAGGiCATAATAATGG - 3′ |  |
SPF2B-bio | F: 5′-GTiGTATCiACAACAGTAACAAA - 3′ |  |
SPF2D-bio | F: 5′-GTiGTATCiACTACAGTAACAAA- 3′ |  |